Citric acid, lactic acid and oxygen metabolism of frozen-thawed semen from four subhuman primate species.

A series of recent studies has been devoted to the systematic examination of characteristics of the semen of subhuman primates, with particular reference to the successful freeze-preservation of these specimens. Roussel & Austin (1967a) showed that trypsin will liquefy the coagulum which appears in these ejaculates without harm to the motility of the sperm cells and the survival rates of cells from five species after 3 days' storage in liquid nitrogen have also been reported (Roussel & Austin, 1967b). The initial content of fructose, lactic acid and citric acid in the frozen semen of animals from eleven species was studied